Plasmodium berghei: Implication of Intracellular Glutathione and Its Related Enzyme in Chloroquine Resistance in Vivo
Identifieur interne : 002B45 ( Main/Exploration ); précédent : 002B44; suivant : 002B46Plasmodium berghei: Implication of Intracellular Glutathione and Its Related Enzyme in Chloroquine Resistance in Vivo
Auteurs : V. L. Dubois [France] ; D. F. N. Platel [France] ; G. Pauly [France] ; J. Tribouleyduret [France]Source :
- Experimental Parasitology [ 0014-4894 ] ; 1995.
Abstract
Abstract: Glutathione (GSH) plays a critical role in the detoxication and the protection of cells against oxidative stress. In the present study we examined the relationship between the intracellular GSH level as well as glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx) activities and how they relate to Plasmodium berghei resistance to chloroquine. Resistant strains (CQR30 and CQR60) were selected in vivo from a sensitive strain (NK65). Marked increases in GSH levels and GST activity within resistant parasites were observed, compared to sensitive parasites. On the other hand, GR and GPx activities were similar in sensitive and resistant parasites. Treatment with chloroquine did not influence the intracellular level of GSH, but it was found to significantly decrease GR activity. Intracellular depletion of GSH, by a nontoxic concentration of buthionine sulfoximine (BSO), significantly sensitized the resistant parasites to chloroquine. These results suggest that the P. berghei resistance results from altered GSH and GST levels and activity, respectively, which enable the detoxification of chloroquine in resistant parasites.
Url:
DOI: 10.1006/expr.1995.1099
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000774
- to stream Istex, to step Curation: 000774
- to stream Istex, to step Checkpoint: 001936
- to stream Main, to step Merge: 002C03
- to stream Main, to step Curation: 002B45
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Plasmodium berghei: Implication of Intracellular Glutathione and Its Related Enzyme in Chloroquine Resistance in Vivo</title><author><name sortKey="Dubois, V L" sort="Dubois, V L" uniqKey="Dubois V" first="V. L." last="Dubois">V. L. Dubois</name></author><author><name sortKey="Platel, D F N" sort="Platel, D F N" uniqKey="Platel D" first="D. F. N." last="Platel">D. F. N. Platel</name></author><author><name sortKey="Pauly, G" sort="Pauly, G" uniqKey="Pauly G" first="G." last="Pauly">G. Pauly</name></author><author><name sortKey="Tribouleyduret, J" sort="Tribouleyduret, J" uniqKey="Tribouleyduret J" first="J." last="Tribouleyduret">J. Tribouleyduret</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CC09C9FC0F0BE631DD888F853A858282EEE03360</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1006/expr.1995.1099</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-CZK44QVW-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000774</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000774</idno><idno type="wicri:Area/Istex/Curation">000774</idno><idno type="wicri:Area/Istex/Checkpoint">001936</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001936</idno><idno type="wicri:doubleKey">0014-4894:1995:Dubois V:plasmodium:berghei:implication</idno><idno type="wicri:Area/Main/Merge">002C03</idno><idno type="wicri:Area/Main/Curation">002B45</idno><idno type="wicri:Area/Main/Exploration">002B45</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Plasmodium berghei: Implication of Intracellular Glutathione and Its Related Enzyme in Chloroquine Resistance in Vivo</title><author><name sortKey="Dubois, V L" sort="Dubois, V L" uniqKey="Dubois V" first="V. L." last="Dubois">V. L. Dubois</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Univ Bordeaux 2, UFR Sci Pharmaceut, Immunol & Parasitol Lab, 146 Leo Saignat, F 33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Platel, D F N" sort="Platel, D F N" uniqKey="Platel D" first="D. F. N." last="Platel">D. F. N. Platel</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Univ Bordeaux 2, UFR Sci Pharmaceut, Immunol & Parasitol Lab, 146 Leo Saignat, F 33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Pauly, G" sort="Pauly, G" uniqKey="Pauly G" first="G." last="Pauly">G. Pauly</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Univ Bordeaux 2, UFR Sci Pharmaceut, Immunol & Parasitol Lab, 146 Leo Saignat, F 33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author><author><name sortKey="Tribouleyduret, J" sort="Tribouleyduret, J" uniqKey="Tribouleyduret J" first="J." last="Tribouleyduret">J. Tribouleyduret</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Univ Bordeaux 2, UFR Sci Pharmaceut, Immunol & Parasitol Lab, 146 Leo Saignat, F 33076 Bordeaux</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Bordeaux</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Parasitology</title><title level="j" type="abbrev">YEXPR</title><idno type="ISSN">0014-4894</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">81</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="117">117</biblScope><biblScope unit="page" to="124">124</biblScope></imprint><idno type="ISSN">0014-4894</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4894</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Glutathione (GSH) plays a critical role in the detoxication and the protection of cells against oxidative stress. In the present study we examined the relationship between the intracellular GSH level as well as glutathione S-transferase (GST), glutathione reductase (GR), and glutathione peroxidase (GPx) activities and how they relate to Plasmodium berghei resistance to chloroquine. Resistant strains (CQR30 and CQR60) were selected in vivo from a sensitive strain (NK65). Marked increases in GSH levels and GST activity within resistant parasites were observed, compared to sensitive parasites. On the other hand, GR and GPx activities were similar in sensitive and resistant parasites. Treatment with chloroquine did not influence the intracellular level of GSH, but it was found to significantly decrease GR activity. Intracellular depletion of GSH, by a nontoxic concentration of buthionine sulfoximine (BSO), significantly sensitized the resistant parasites to chloroquine. These results suggest that the P. berghei resistance results from altered GSH and GST levels and activity, respectively, which enable the detoxification of chloroquine in resistant parasites.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Bordeaux</li></settlement></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Dubois, V L" sort="Dubois, V L" uniqKey="Dubois V" first="V. L." last="Dubois">V. L. Dubois</name></region><name sortKey="Pauly, G" sort="Pauly, G" uniqKey="Pauly G" first="G." last="Pauly">G. Pauly</name><name sortKey="Platel, D F N" sort="Platel, D F N" uniqKey="Platel D" first="D. F. N." last="Platel">D. F. N. Platel</name><name sortKey="Tribouleyduret, J" sort="Tribouleyduret, J" uniqKey="Tribouleyduret J" first="J." last="Tribouleyduret">J. Tribouleyduret</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B45 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002B45 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CC09C9FC0F0BE631DD888F853A858282EEE03360
|texte= Plasmodium berghei: Implication of Intracellular Glutathione and Its Related Enzyme in Chloroquine Resistance in Vivo
}}
| This area was generated with Dilib version V0.6.33. |  |